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NG2 expression at levels of the mRNA, intracellular protein, and secreted protein in BMDMΦ treated with myelin debris. (A) NG2 mRNA expression in BMDMΦ treated with myelin debris for 7 days. (B) NG2 protein expression in BMDMΦ treated with myelin debris for 7 days and 14 days assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed twice with similar results. C: control; T: myelin debris treatment. (C) NG2 protein expression in microglia with myelin debris for indicated time points assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed four times with similar results. C: control; T: myelin debris treatment. (D) Chondroitin sulfate proteoglycan <t>4</t> <t>(CSPG4)</t> in the supernatant of BMDMΦ treated with myelin debris for 7 days and 14 days detected by Enzyme-linked immunosorbent assay <t>(ELISA).</t> Data for all quantifications are shown as means ± SD of three separate BMDMΦ isolations from three separate mice ( n = 3). ** P ≤ 0.01.
Quantitative Sandwich Elisa Kit G Biosciences Cat. #It5412, supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative sandwich elisa kit mbs7607909  (MyBiosource Biotechnology)
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NG2 expression at levels of the mRNA, intracellular protein, and secreted protein in BMDMΦ treated with myelin debris. (A) NG2 mRNA expression in BMDMΦ treated with myelin debris for 7 days. (B) NG2 protein expression in BMDMΦ treated with myelin debris for 7 days and 14 days assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed twice with similar results. C: control; T: myelin debris treatment. (C) NG2 protein expression in microglia with myelin debris for indicated time points assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed four times with similar results. C: control; T: myelin debris treatment. (D) Chondroitin sulfate proteoglycan <t>4</t> <t>(CSPG4)</t> in the supernatant of BMDMΦ treated with myelin debris for 7 days and 14 days detected by Enzyme-linked immunosorbent assay <t>(ELISA).</t> Data for all quantifications are shown as means ± SD of three separate BMDMΦ isolations from three separate mice ( n = 3). ** P ≤ 0.01.
Quantitative Sandwich Elisa Kit Mbs7607909, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative sandwich rat cyclic guanosine monophosphate (c-gmp) elisa kit  (MyBiosource Biotechnology)
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MyBiosource Biotechnology quantitative sandwich rat cyclic guanosine monophosphate (c-gmp) elisa kit
Estimated levels of blood glucose, LDH, amylase, MPO, and TNF-α in all studied groups ( n = 6 in each group). p -value ≤0.05 is considered significant.
Quantitative Sandwich Rat Cyclic Guanosine Monophosphate (C Gmp) Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative sandwich elisa kit mbs021959  (MyBiosource Biotechnology)
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Estimated levels of blood glucose, LDH, amylase, MPO, and TNF-α in all studied groups ( n = 6 in each group). p -value ≤0.05 is considered significant.
Quantitative Sandwich Elisa Kit Mbs021959, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NG2 expression at levels of the mRNA, intracellular protein, and secreted protein in BMDMΦ treated with myelin debris. (A) NG2 mRNA expression in BMDMΦ treated with myelin debris for 7 days. (B) NG2 protein expression in BMDMΦ treated with myelin debris for 7 days and 14 days assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed twice with similar results. C: control; T: myelin debris treatment. (C) NG2 protein expression in microglia with myelin debris for indicated time points assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed four times with similar results. C: control; T: myelin debris treatment. (D) Chondroitin sulfate proteoglycan 4 (CSPG4) in the supernatant of BMDMΦ treated with myelin debris for 7 days and 14 days detected by Enzyme-linked immunosorbent assay (ELISA). Data for all quantifications are shown as means ± SD of three separate BMDMΦ isolations from three separate mice ( n = 3). ** P ≤ 0.01.

Journal: Frontiers in Cellular Neuroscience

Article Title: Myelin Debris Stimulates NG2/CSPG4 Expression in Bone Marrow-Derived Macrophages in the Injured Spinal Cord

doi: 10.3389/fncel.2021.651827

Figure Lengend Snippet: NG2 expression at levels of the mRNA, intracellular protein, and secreted protein in BMDMΦ treated with myelin debris. (A) NG2 mRNA expression in BMDMΦ treated with myelin debris for 7 days. (B) NG2 protein expression in BMDMΦ treated with myelin debris for 7 days and 14 days assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed twice with similar results. C: control; T: myelin debris treatment. (C) NG2 protein expression in microglia with myelin debris for indicated time points assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed four times with similar results. C: control; T: myelin debris treatment. (D) Chondroitin sulfate proteoglycan 4 (CSPG4) in the supernatant of BMDMΦ treated with myelin debris for 7 days and 14 days detected by Enzyme-linked immunosorbent assay (ELISA). Data for all quantifications are shown as means ± SD of three separate BMDMΦ isolations from three separate mice ( n = 3). ** P ≤ 0.01.

Article Snippet: CSPG4 protein in BMDMΦ was quantified by a commercial quantitative sandwich ELISA kit (G-Biosciences Cat. #IT5412).

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay

Estimated levels of blood glucose, LDH, amylase, MPO, and TNF-α in all studied groups ( n = 6 in each group). p -value ≤0.05 is considered significant.

Journal: Frontiers in Physiology

Article Title: Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury

doi: 10.3389/fphys.2022.934597

Figure Lengend Snippet: Estimated levels of blood glucose, LDH, amylase, MPO, and TNF-α in all studied groups ( n = 6 in each group). p -value ≤0.05 is considered significant.

Article Snippet: Then using quantitative Sandwich Rat Cyclic Guanosine Monophosphate (c-GMP) ELISA Kit (MyBioSource, Catalog #MBS007871, the level of c-GMP was estimated and expressed per ml.

Techniques: